• Premise of study: Microsatellite primers were developed for castor bean (Ricinus communis L.) to investigate genetic diversity and population structure, and to provide support to germplasm management.
• Methods and Results: Eleven microsatellite loci were isolated using an enrichment cloning protocol and used to characterize castor bean germplasm from the collection at the Instituto Agronômico de Campinas (IAC). In a survey of 76 castor bean accessions, the investigated loci displayed polymorphism ranging from two to five alleles.
• Conclusions: The information derived from microsatellite markers led to significant gains in conserved allelic richness and provides support to the implementation of several molecular breeding strategies for castor bean.
American Journal of Botany (2011)
AJB Primer Notes & Protocols in the Plant Sciences
DOI:10.3732/ajb.1000395
Miklos Maximiliano Bajay, Maria Imaculada Zucchi, Tammy Aparecida Manabe KIIhl, Carlos Eduardo Araújo Batista, Mariza Monteiro and José Baldin Pinheiro
Development and characterization of microsatellite markers for castor (Ricinus communis L.), an important oleaginous species for biodiesel production
Castor (Ricinus communis L.) is an important oleaginous plant from both economic and social points of view. The seeds contain an oil with excellent properties for industrial uses. This paper presents the main results of a study aiming to develop microsatellite markers for castor. Twelve new polymorphic microsatellite markers were isolated and characterized in 38 genotypes accessions from the castor germplasm of the Brazilian Agricultural Research Company (EMBRAPA). Knowledge on the genetic diversity of castor can be used to gain a better understanding on genetic diversity conservation, and germplasm management, guiding breeding programs and conservation strategies.
CONSERVATION GENETICS RESOURCES (2009)
Volume 1, Number 1, 237-239, DOI: 10.1007/s12686-009-9058-z
Miklos Maximiliano Bajay, José Baldin Pinheiro, Carlos Eduardo Araújo Batista,Márcia Barreto Medeiros Nobrega, Maria Imaculada Zucchi
Desenvolvimento de marcadores microssatélites e caracterização do germoplasma de mamona (Ricinus communis L.)
The castor bean (Ricinus communis L.) is an oil plant with a high economic and social value in Brazil and over the world. The seeds contain oil with excellent properties for industrial uses. It is the only vegetable oil soluble in alcohol, presenting high viscosity and requiring less heating than other oils during the production of biodiesel. Castor diversity is well represented in Brazilian germplasm collections, but there is little information on the molecular characterization of the accessions, and diversity loss is a serious risk. The success in a breeding program depends on the knowledge about the genetic diversity of the available germplasm. Microsattelite is an important tool for estimating the Genetic diversity, This study aims to develop microsatellite markers from an enriched microsatellite DNA library for castor and characterize in genotypes accessions from the castor germplasm of the Brazilian Agricultural Research Company (EMBRAPA), castor germplasm of the Agronomic Institute at Campinas (IAC) and genotypes from State University of São Paulo (UNESP) - Botucatu. From the 41 loci microsatellites analyzed, 26 showed polymorphism revealing 111 alleles independent (4,27 alleles by loco). 37 alleles have presented an inferior frequency 5%, being considered as rare alleles. 41 alleles are particular, being observed in only one of the three analyzed populations. The average allelic richness was 2,554. The average HO=0,054 was smaller than the average HE=0,473, evidencing a heterozygote deficit (average GIS=0,845). The samples has shown a strong structure with a significant differentiation (GST'= 0.364). The Twenty six loci differ significantly from Hardy Weinberg Equilibrium (P<5%) after Bonferroni correction. The Results of STRUCTURE graphic, main component analysis and dendrograms shows the formation of two genetically distinct groups, a group is composed by the accesses of the EMBRAPA and the other group is composed by the accesses of the IAC and the genotypes of UNESP - Botucatu. This fact indicates that the genetic diversity of castor is well represented. Knowledge on the genetic diversity of castor can be used to gain a better understanding on genetic diversity conservation, and germplasm management, guiding breeding programs and conservation strategies.
Miklos Maximiliano Bajay's master dissertation (USP-ESALQ)
Genetic and Plant Breeding department
